Overview

  • Product name
  • Description
    Rabbit polyclonal to ZC3H8
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 50 and 100 of human ZC3H8 using the numbering given in entry NP_115883.2 (GeneID 84524).

  • Positive control
    • Whole cell lysate from HeLa or 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab113260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 34 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

Images

  • All lanes : Anti-ZC3H8 antibody (ab113260) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 34 kDa


    Exposure time : 3 minutes
  • Lane 1: 1mg whole cell lysate from HeLa cells was immunoprecipitated with ab113260 at 6µg/mg lysate and 20% IP was loaded on gel.
    Lane 2: same, with a control IgG.
    For WB ab113260 was used at 1µg/ml.
    Detection: chemiluminescence with exposure time of 10 seconds.

References

ab113260 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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