Overview

  • Product nameAnti-ZCCHC12 antibody
    See all ZCCHC12 primary antibodies
  • Description
    Rabbit polyclonal to ZCCHC12
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: AREVMRVLQA TNPNLSVADF LRAMKLVFGE SESSVTAHGK FFNTLQAQGE, corresponding to amino acids 94-143 of Human ZCCHC12

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab74774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionTranscriptional coactivator in the bone morphogenetic protein (BMP)-signaling pathway. It positively modulates BMP signaling by interacting with SMAD1 and associating with CBP in the transcription complex. It contributes to the BMP-induced enhancement of cholinergic-neuron-specific gene expression.
  • Sequence similaritiesBelongs to the ZCCHC12 family.
    Contains 1 CCHC-type zinc finger.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810028A01Rik antibody
    • FLJ16123 antibody
    • Paraneoplastic Ma antigen family member 7A antibody
    • PNMA7A antibody
    • SIZN antibody
    • SIZN1 antibody
    • Smad interacting zinc finger protein 1 antibody
    • Smad-interacting zinc finger protein 1 antibody
    • Zcchc12 antibody
    • ZCH12_HUMAN antibody
    • Zinc finger CCHC domain containing 12 antibody
    • Zinc finger CCHC domain containing protein 12 antibody
    • Zinc finger CCHC domain-containing protein 12 antibody
    see all

Anti-ZCCHC12 antibody images

  • Anti-ZCCHC12 antibody (ab74774) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ZCCHC12 antibody (ab74774)

ab74774 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (bone marrow derived mesenchymal stem cell)
Loading amount 15 µg
Specification bone marrow derived mesenchymal stem cell
Treatment 20 uM specific compound for 48hrs
Gel Running Conditions Reduced Denaturing (gel 10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Sep 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"