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Rabbit polyclonal to ZCCHC16
Recombinant fragment, corresponding to amino acids 161-309 of Human ZCCHC16 (Q6ZR62).
- RT4, U-251 MG, Human Liver and Human Tonsil lysates; Human colon tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Contains 1 CCHC-type zinc finger.
Information by UniProt
- Mammalian retrotransposon-derived protein 4 antibody
- Zcchc16 antibody
- ZCH16_HUMAN antibody
- Zinc finger CCHC domain-containing protein 16 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC16 antibody (ab121184)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121184 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-ZCCHC16 antibody (ab121184)
All lanes : Anti-ZCCHC16 antibody (ab121184) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC16 antibody (ab121184)
ab121184 at 1/250 dilution staining ZCCHC16 in Paraffin-emebedded Human colon tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"