Anti-ZCCHC4 antibody (ab154002)
Key features and details
- Rabbit polyclonal to ZCCHC4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZCCHC4 antibody
See all ZCCHC4 primary antibodies -
Description
Rabbit polyclonal to ZCCHC4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to a region within amino acids 2-246 of Human ZCCHC4 (UniProt ID: Q9H5U6).
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Positive control
- 293T, A431, Jurkat and Raji whole cell lysates; A431 cells; Human PC13 xenograft tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 59 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 59 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
May be a methyltransferase. -
Sequence similarities
Contains 1 CCHC-type zinc finger.
Contains 1 DHHC-type zinc finger. - Information by UniProt
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Database links
- Entrez Gene: 511397 Cow
- Entrez Gene: 29063 Human
- Omim: 611792 Human
- SwissProt: E1BGQ2 Cow
- SwissProt: Q9H5U6 Human
- Unigene: 278945 Human
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Alternative names
- 4930449I23Rik antibody
- DHHC domain containing zinc finger protein antibody
- HSPC052 antibody
see all
Images
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Anti-ZCCHC4 antibody (ab154002) at 1/3000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 59 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde fixed A431 cells labeling ZCCHC4 with ab154002 at 1/500 dilution. Lower image costained with Hoerchst 33342.
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Immunohistochemical analysis of paraffin embedded Human PC13 xenograft tissue, labeling ZCCHC4 with ab154002 antibody at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154002 has not yet been referenced specifically in any publications.