Overview

  • Product name
  • Description
    Rabbit polyclonal to ZCCHC4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 2-246 of Human ZCCHC4 (UniProt ID: Q9H5U6).

  • Positive control
    • 293T, A431, Jurkat and Raji whole cell lysates; A431 cells; Human PC13 xenograft tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    May be a methyltransferase.
  • Sequence similarities
    Contains 1 CCHC-type zinc finger.
    Contains 1 DHHC-type zinc finger.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930449I23Rik antibody
    • DHHC domain containing zinc finger protein antibody
    • HSPC052 antibody
    • MGC21108 antibody
    • OTTMUSP00000027189 antibody
    • zcchc4 antibody
    • ZCHC4_HUMAN antibody
    • ZGRF4 antibody
    • Zinc finger CCHC domain containing protein 4 antibody
    • Zinc finger CCHC domain-containing protein 4 antibody
    • Zinc finger CCHC type containing 4 antibody
    • Zinc finger GRF type containing 4 antibody
    • Zinc finger, CCHC domain containing 4 antibody
    see all

Images

  • Anti-ZCCHC4 antibody (ab154002) at 1/3000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 59 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde fixed A431 cells labeling ZCCHC4 with ab154002 at 1/500 dilution. Lower image costained with Hoerchst 33342.
  • Immunohistochemical analysis of paraffin embedded Human PC13 xenograft tissue, labeling ZCCHC4 with ab154002 antibody at 1/500 dilution.

References

ab154002 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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