Overview

  • Product nameAnti-ZCCHC6 antibody
  • Description
    Mouse polyclonal to ZCCHC6
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human ZCCHC6 protein (aa 1-412 of AAH32456 or Swissprot id Q5VYS8-5).

  • Positive control
    • Human liver tissue lysate; ZCCHC6 transfected 293T cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesIHC-P: 1/50.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight, isoform 1: 171 kDa, isoform 5: 48 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceZCCHC6 contains 3 CCHC-type zinc fingers.
    • Database links
    • Alternative names
      • KIAA1711 antibody
      • MGC38585 antibody
      • PAP associated domain containing 6 antibody
      • PAPD6 antibody
      • Zinc finger CCHC domain containing 6 antibody
      see all

    Anti-ZCCHC6 antibody images

    • Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml + human liver tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 171 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml

      Lane 1 : ZCCHC6 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 171 kDa
      Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    • ab76901 staining ZCCHC6 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent antigen retrieval in 0.1 sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining method was used for detection. 

    References for Anti-ZCCHC6 antibody (ab76901)

    ab76901 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"