Overview

  • Product name
  • Description
    Mouse polyclonal to ZCCHC8
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    ZCCHC8 (NP_060082, 1 a.a. ~ 707 a.a) full-length human protein.

  • Positive control
    • ZCCHC8 transfected 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 79 kDa).

Target

  • Function
    May be involved in pre-mRNA splicing.
  • Sequence similarities
    Belongs to the ZCCHC8 family.
    Contains 1 CCHC-type zinc finger.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434E2220 antibody
    • ZCCHC8 antibody
    • ZCHC8_HUMAN antibody
    • Zinc finger CCHC domain containing 8 antibody
    • Zinc finger CCHC domain containing protein 8 antibody
    • Zinc finger CCHC domain-containing protein 8 antibody
    see all

Anti-ZCCHC8 antibody images

  • All lanes : Anti-ZCCHC8 antibody (ab68739) at 1/500 dilution

    Lane 1 : ZCCHC8 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 79 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)

References for Anti-ZCCHC8 antibody (ab68739)

This product has been referenced in:
  • Falk S  et al. Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors. Nat Commun 7:13573 (2016). WB . Read more (PubMed: 27905398) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
50 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Mar 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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