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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 79 kDa).
Anti-ZCCHC8 antibody images
Western blot - ZCCHC8 antibody (ab68739)
All lanes :
Anti-ZCCHC8 antibody (ab68739) at 1/500 dilutionLane 1 :
ZCCHC8 transfected 293T cell lysateLane 2 :
Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionPredicted band size :
79 kDaObserved band size :
110 kDa (why is the actual band size different from the predicted?
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