Overview

  • Product nameAnti-ZCCHC8 antibody
    See all ZCCHC8 primary antibodies
  • Description
    Mouse polyclonal to ZCCHC8
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    ZCCHC8 (NP_060082, 1 a.a. ~ 707 a.a) full-length human protein.

  • Positive control
    • ZCCHC8 transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 79 kDa).

Target

  • FunctionMay be involved in pre-mRNA splicing.
  • Sequence similaritiesBelongs to the ZCCHC8 family.
    Contains 1 CCHC-type zinc finger.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434E2220 antibody
    • ZCCHC8 antibody
    • ZCHC8_HUMAN antibody
    • Zinc finger CCHC domain containing 8 antibody
    • Zinc finger CCHC domain containing protein 8 antibody
    • Zinc finger CCHC domain-containing protein 8 antibody
    see all

Anti-ZCCHC8 antibody images

  • All lanes : Anti-ZCCHC8 antibody (ab68739) at 1/500 dilution

    Lane 1 : ZCCHC8 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 79 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)

References for Anti-ZCCHC8 antibody (ab68739)

ab68739 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS cells)
Loading amount 50 µg
Specification U2OS cells
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"