Overview

  • Product name
    Anti-ZCCHC9 antibody
  • Description
    Rabbit polyclonal to ZCCHC9
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: TTYNKRPLPA TSWEDMKKGS FEGTSQNLPK RKQLEANRLS LKNDAPQAKH KKNKKKKEYL NEDVNGFMEY LRQNSQMVHN GQI, corresponding to N terminal amino acids 9-91 of Human ZCCHC9.

  • Positive control
    • Human duodenum tissue; ZCCHC9 over-expression HEK293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab122470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Sequence similarities
    Contains 4 CCHC-type zinc fingers.
  • Information by UniProt
  • Database links
  • Alternative names
    • PPP1R41 antibody
    • Protein phosphatase 1 regulatory subunit 41 antibody
    • Zcchc9 antibody
    • ZCHC9_HUMAN antibody
    • Zinc finger CCHC domain containing protein 9 antibody
    • Zinc finger CCHC domain-containing protein 9 antibody
    • Zinc finger, CCHC domain containing 9 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and nucleoli. Recommended concentration of ab122470 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122470, at 1/500 dilution, staining ZCCHC9 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
  • All lanes : Anti-ZCCHC9 antibody (ab122470) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag [~3.1 kDa] in HEK293T cells)

    Developed using the ECL technique

References

ab122470 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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