Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab89165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 185 kDa.

Target

  • Function
    Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation.
  • Sequence similarities
    Belongs to the DNA polymerase type-B-like family.
    Contains 3 CCHC-type zinc fingers.
    Contains 2 PAP-associated domains.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Cytoplasm. Translocates into the cytoplasm following treatment of the cell with LPS.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp779C1943 antibody
    • EC 2.7.7.52 antibody
    • FLJ42878 antibody
    • KIAA0191 antibody
    • PAP associated domain containing 3 antibody
    • PAPD3 antibody
    • Terminal uridylyltransferase 4 antibody
    • TUT4 antibody
    • TUT4_HUMAN antibody
    • TUTase 4 antibody
    • ZCCHC 11 antibody
    • Zcchc11 antibody
    • Zinc finger CCHC domain containing 11 antibody
    • Zinc finger CCHC domain containing protein 11 antibody
    • Zinc finger CCHC domain-containing protein 11 antibody
    see all

Images

  • Anti-ZCH11 antibody (ab89165) at 1 µg/ml + Human spleen tissue lysate at 50 µg

    Predicted band size : 185 kDa
    Observed band size : 185 kDa
  • All lanes : Anti-ZCH11 antibody (ab89165) at 1 µg/ml

    Lane 1 : ZCH11 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 185 kDa

References

This product has been referenced in:

See 1 Publication for this product

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