The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Can be blocked with Human ZCH11 peptide (ab23172). Approx 250-300 kDa and 30-35 kDa band seen in Raji Lysate. Please note that the band we consistently detect in western blots differs from the predicted size of 181kDa. Currently we cannot find an explanation for this in the literature. However, it has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE.
Use at an assay dependent dilution. In paraffin-embedded Human Lymph Node shows nuclear staining of select lymphocytes and staining of macrophages.
FunctionUridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation.
Sequence similaritiesBelongs to the DNA polymerase type-B-like family. Contains 3 CCHC-type zinc fingers. Contains 2 PAP-associated domains.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm. Translocates into the cytoplasm following treatment of the cell with LPS.