Overview

  • Product name
  • Description
    Goat polyclonal to ZCH11
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Predicted to work with: Mouse, Rat, Cow, Dog, Human
  • Immunogen

    Synthetic peptide:

    RERCPHPPRGNVSE

    , corresponding to C terminal amino acids 1503-1516 of KIAA0191 (protein sequence according to BAA12105)

  • Positive control
    • Human lymph Node
  • General notes
    Principal Names - KIAA0191 protein GenBank Accession Number – BAA12105 LocusLink ID - 23318 Note:Please Click Here for Instructions For Use/Protocol


    A ubiquitously expressed gene with a predicted C2H2 zinc-finger at its N-terminal region.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes
    A ubiquitously expressed gene with a predicted C2H2 zinc-finger at its N-terminal region.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with Human ZCH11 peptide (ab23172). Approx 250-300 kDa and 30-35 kDa band seen in Raji Lysate. Please note that the band we consistently detect in western blots differs from the predicted size of 181kDa. Currently we cannot find an explanation for this in the literature. However, it has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE.
IHC-P Use at an assay dependent dilution. In paraffin-embedded Human Lymph Node shows nuclear staining of select lymphocytes and staining of macrophages.

Target

  • Function
    Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation.
  • Sequence similarities
    Belongs to the DNA polymerase type-B-like family.
    Contains 3 CCHC-type zinc fingers.
    Contains 2 PAP-associated domains.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Cytoplasm. Translocates into the cytoplasm following treatment of the cell with LPS.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp779C1943 antibody
    • EC 2.7.7.52 antibody
    • FLJ42878 antibody
    • KIAA0191 antibody
    • PAP associated domain containing 3 antibody
    • PAPD3 antibody
    • Terminal uridylyltransferase 4 antibody
    • TUT4 antibody
    • TUT4_HUMAN antibody
    • TUTase 4 antibody
    • ZCCHC 11 antibody
    • Zcchc11 antibody
    • Zinc finger CCHC domain containing 11 antibody
    • Zinc finger CCHC domain containing protein 11 antibody
    • Zinc finger CCHC domain-containing protein 11 antibody
    see all

Anti-ZCH11 antibody images

  • ab9940 staining ZCH11 in Human Lymph Node at 4 µg/ml. Steamed antigen retrieval with citrate buffer pH 6 was performed, antibody revealed with HRP-staining

References for Anti-ZCH11 antibody (ab9940)

ab9940 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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