General notesab94144 is a 293T cell transfected lysate in which Human ZCH11 has been transiently over-expressed using a pCMV-ZCH11 plasmid. The lysate is provided in 1 x Sample Buffer.
Note: For more details about how the transfected lysate was prepared view preparation notes
BackgroundFunction: Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation.
Similarity: Belongs to the DNA polymerase type-B-like family.
Contains 3 CCHC-type zinc fingers.
Contains 2 PAP-associated domains.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.