Anti-zcrb1 antibody - C-terminal (ab171439)

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Overview

  • Product name
    Anti-zcrb1 antibody - C-terminal
    See all zcrb1 primary antibodies
  • Description
    Rabbit polyclonal to zcrb1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human zcrb1 aa 183-212 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8TBF4

  • Positive control
    • Mouse Neuro-2a cells and lysate; Human stomach tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171439 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab171439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 25 kDa.

Target

  • Sequence similarities
    Contains 1 CCHC-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization
    Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • MADP 1 antibody
      • MADP1 antibody
      • RBM36 antibody
      • SNRNP31 antibody
      • U11/U12 small nuclear ribonucleoprotein 31 kDa protein antibody
      • U11/U12 snRNP 31 kDa protein antibody
      • U11/U12 snRNP 31K antibody
      • U11/U12-31K antibody
      • ZCCHC19 antibody
      • Zcrb1 antibody
      • ZCRB1_HUMAN antibody
      • zinc finger CCHC-type and RNA binding motif 1 antibody
      • Zinc finger CCHC-type and RNA-binding motif-containing protein 1 antibody
      see all

    Images

    • Western blot - Anti-zcrb1 antibody - C-terminal (ab171439)
      Western blot - Anti-zcrb1 antibody - C-terminal (ab171439)
      Anti-zcrb1 antibody - C-terminal (ab171439) at 1/100 dilution + Mouse Neuro-2a cell line lysate at 35 µg

      Predicted band size: 25 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-zcrb1 antibody - C-terminal (ab171439)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-zcrb1 antibody - C-terminal (ab171439)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue labeling zcrb1 with ab171439 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

    • Flow Cytometry - Anti-zcrb1 antibody - C-terminal (ab171439)
      Flow Cytometry - Anti-zcrb1 antibody - C-terminal (ab171439)

      Flow cytometric analysis of Neuro-2a cells labeling zcrb1 with ab171439 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC conjugated donkey anti-rabbit secondary antibodies were used for the analysis.

    References

    ab171439 has not yet been referenced specifically in any publications.

    Publishing research using ab171439? Please let us know so that we can cite the reference in this datasheet.

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