Anti-zcrb1 antibody - C-terminal (ab171439)

Overview

  • Product name
    Anti-zcrb1 antibody - C-terminal
    See all zcrb1 primary antibodies
  • Description
    Rabbit polyclonal to zcrb1 - C-terminal
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human zcrb1 aa 183-212 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8TBF4

  • Positive control
    • Mouse Neuro-2a cells and lysate; Human stomach tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171439 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab171439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 25 kDa.

Target

  • Sequence similarities
    Contains 1 CCHC-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization
    Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • MADP 1 antibody
    • MADP1 antibody
    • RBM36 antibody
    • SNRNP31 antibody
    • U11/U12 small nuclear ribonucleoprotein 31 kDa protein antibody
    • U11/U12 snRNP 31 kDa protein antibody
    • U11/U12 snRNP 31K antibody
    • U11/U12-31K antibody
    • ZCCHC19 antibody
    • Zcrb1 antibody
    • ZCRB1_HUMAN antibody
    • zinc finger CCHC-type and RNA binding motif 1 antibody
    • Zinc finger CCHC-type and RNA-binding motif-containing protein 1 antibody
    see all

Images

  • Anti-zcrb1 antibody - C-terminal (ab171439) at 1/100 dilution + Mouse Neuro-2a cell line lysate at 35 µg

    Predicted band size : 25 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue labeling zcrb1 with ab171439 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of Neuro-2a cells labeling zcrb1 with ab171439 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC conjugated donkey anti-rabbit secondary antibodies were used for the analysis.

References

ab171439 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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