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Rabbit polyclonal to ZCWPW2
antigen sequence: TATPDESEEG HGEEINMGEK LSKCSPEAPA GSLFENHYEE DYLVIDGIKL KAGECIEDIT NKFKEIDALM SEF, corresponding to amino acids 284-356 of Human ZCWPW2.
Human adrenal gland tissue; RT4, U251 MG and Human liver lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/250 - 1/500. Predicted molecular weight: 41 kDa.
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Contains 1 CW-type zinc finger.
Contains 1 PWWP domain.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-ZCWPW2 antibody (ab126482)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, vesicles and cytoskeleton (microtubules). Recommended concentration of ab126482 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCWPW2 antibody (ab126482)
ab126482 at a 1/500 dilution staining ZCWPW2 in paraffin embedded Human adrenal gland tissue by immunohistochemistry.
Western blot - Anti-ZCWPW2 antibody (ab126482)
All lanes : Anti-ZCWPW2 antibody (ab126482) at 1/250 dilution Lane 1 : RT4 cell lysate Lane 2 : U251 MG cell lysate Lane 3 : Human plasma lysate Lane 4 : Human liver tissue lysate Lane 5 : Human tonsil tissue lysate Predicted band size: 41 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"