Overview

  • Product name
    Anti-ZDHHC15 antibody
  • Description
    Rabbit polyclonal to ZDHHC15
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: VSRNKTTLEA FCTPVFTSGP EKNGFNLGFI KNIQQVFGDK KKFWLIPIGS SPGDGHSFPM RSMNESQNPL LANEETWEDN EDDNQ, corresponding to internal sequence amino acids 238-322 of Human ZDHHC15.

  • Positive control
    • Human pancreas tissue; Human liver lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Palmitoyltransferase specific for GAP43 and DLG4/PSD95.
  • Tissue specificity
    Expressed in placenta, liver, lung, kidney, heart and brain.
  • Involvement in disease
    Defects in ZDHHC15 are the cause of mental retardation X-linked type 91 (MRX91) [MIM:300577]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
  • Sequence similarities
    Belongs to the DHHC palmitoyltransferase family.
    Contains 1 DHHC-type zinc finger.
  • Domain
    The DHHC domain is required for palmitoyltransferase activity.
  • Post-translational
    modifications
    Autopalmitoylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DHHC-15 antibody
    • Palmitoyltransferase ZDHHC15 antibody
    • UNQ1969/PRO4501 antibody
    • ZDH15_HUMAN antibody
    • Zdhhc15 antibody
    • Zinc finger DHHC domain-containing protein 15 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121203 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121203 at 1/5 dilution staining ZDHHC15 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.
  • All lanes : Anti-ZDHHC15 antibody (ab121203) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Human Liver cell lysate
    Lane 5 : Human tonsil cell lysate

References

ab121203 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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