Anti-ZDHHC20 antibody - N-terminal (ab171838)

Overview

  • Product name
    Anti-ZDHHC20 antibody - N-terminal
    See all ZDHHC20 primary antibodies
  • Description
    Rabbit polyclonal to ZDHHC20 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within ZDHHC20 aa 25-54 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q5W0Z9

  • Positive control
    • NCI-H460 cell line lysates. Human stomach tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99.91% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171838 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 42 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Belongs to the DHHC palmitoyltransferase family.
    Contains 1 DHHC-type zinc finger.
  • Domain
    The DHHC domain is required for palmitoyltransferase activity.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4933421L13Rik antibody
    • DHHC-20 antibody
    • Probable palmitoyltransferase ZDHHC20 antibody
    • ZDH20_HUMAN antibody
    • ZDHHC20 antibody
    • Zinc finger DHHC domain-containing protein 20 antibody
    • Zinc finger DHHC type containing 20 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed and paraffin embedded Human stomach tissue labeling ZDHHC20 with ab171838 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-ZDHHC20 antibody - N-terminal (ab171838) at 1/100 dilution + NCI-H460 cell lysate at 35 µg

    Predicted band size: 42 kDa

References

ab171838 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab171838.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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