Overview

  • Product name
  • Description
    Goat polyclonal to ZDHHC8
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide:

    C-QRDHPQLKTPPSK

    , corresponding to internal sequence amino acids 708-720 of Human ZDHHC8

  • Positive control
    • Human Brain (Frontal Cortex) lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab59483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Peptide ELISA: antibody detection limit dilution 1:16,000.

    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 81 kDa). An additional band of 48kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Palmitoyltransferase involved in glutamatergic transmission. Mediates palmitoylation of ABCA1.
    • Sequence similarities
      Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.
      Contains 1 DHHC-type zinc finger.
    • Domain
      The DHHC domain is required for palmitoyltransferase activity.
    • Cellular localization
      Cytoplasmic vesicle membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • DHHC 8 antibody
      • DHHC domain containing 8 antibody
      • DHHC-8 antibody
      • KIAA1292 antibody
      • Membrane associated DHHC8 zinc finger protein antibody
      • Probable palmitoyltransferase ZDHHC8 antibody
      • ZDHC8_HUMAN antibody
      • ZDHHC8 antibody
      • ZDHHCL1 antibody
      • Zinc finger DHHC domain containing protein 8 antibody
      • Zinc finger DHHC domain-containing protein 8 antibody
      • Zinc finger protein 378 antibody
      • ZNF378 antibody
      see all

    Images

    • All lanes : Anti-ZDHHC8 antibody (ab59483) at 0.1 µg/ml

      Lane 1 : Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer) without blocking with the immunising peptide
      Lane 2 : Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer) with blocking with the immunising peptide

      Predicted band size: 81 kDa
      Observed band size: 80 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.



      Primary incubation was 1 hour. Detected by chemiluminescence.

    References

    ab59483 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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