Overview

  • Product nameAnti-ZDHHC9 antibody
    See all ZDHHC9 primary antibodies
  • Description
    Rabbit polyclonal to ZDHHC9
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from C-terminal region of human ZDHHC9.

  • Positive control
    • human brain tissue, extracts from COLO cells. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab74504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionThe ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.
  • Tissue specificityHighly expressed in kidney, skeletal muscle, brain, lung and liver. Absent in thymus, spleen and leukocytes.
  • Involvement in diseaseDefects in ZDHHC9 are the cause of mental retardation syndromic X-linked ZDHHC9-related (MRXSZ) [MIM:300799]. A disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Some patients have marfanoid habitus as an additional feature.
  • Sequence similaritiesBelongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.
    Contains 1 DHHC-type zinc finger.
  • DomainThe DHHC domain is required for palmitoyltransferase activity.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen MMSA 1 antibody
    • Asp His His Cys domain containing protein 9 antibody
    • CGI89 antibody
    • CXorf11 antibody
    • DHHC-9 antibody
    • DHHC9 antibody
    • MMSA1 antibody
    • MRXSZ antibody
    • Palmitoyltransferase ZDHHC9 antibody
    • ZDHC9_HUMAN antibody
    • ZDHHC 9 antibody
    • ZDHHC10 antibody
    • ZDHHC9 antibody
    • Zinc finger DHHC domain containing 10 antibody
    • Zinc finger DHHC domain containing protein 9 antibody
    • Zinc finger DHHC domain-containing protein 9 antibody
    • Zinc finger DHHC type containing 9 antibody
    • Zinc finger protein 379 antibody
    • Zinc finger protein 380 antibody
    • ZNF379 antibody
    • ZNF380 antibody
    see all

Anti-ZDHHC9 antibody images

  • ICC/IF image of ab74504 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab74504 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-ZDHHC9 antibody (ab74504) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 26 kDa,34 kDa,48 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab74504. Left: untreated sample. Right: sample treated with immunising peptide.

References for Anti-ZDHHC9 antibody (ab74504)

This product has been referenced in:
  • Cambronne XA  et al. Capturing microRNA targets using an RNA-induced silencing complex (RISC)-trap approach. Proc Natl Acad Sci U S A 109:20473-8 (2012). WB . Read more (PubMed: 23184980) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"