Overview

  • Product name
    Anti-ZEB2 antibody - C-terminal
    See all ZEB2 primary antibodies
  • Description
    Rabbit Polyclonal to ZEB2 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Smad Interacting Protein 1 aa 1085-1113 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O60315

  • Positive control
    • MDA-MB435 cell lysate, MDA-MB435 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 136 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.
  • Involvement in disease
    Defects in ZEB2 are the cause of Mowat-Wilson syndrome (MWIS) [MIM:235730]; also known as Hirschsprung disease-mental retardation syndrome. A complex developmental disorder characterized by mental retardation, delayed motor development, epilepsy, microcephaly and a wide spectrum of clinically heterogeneous features suggestive of neurocristopathies at the cephalic, cardiac, and vagal levels. Some patients manifest Hirschsprung disease. Affected patients show an easily recognizable facial appearance with deep set eyes and hypertelorism, medially divergent, broad eyebrows, prominent columella, pointed chin and uplifted, notched ear lobes.
  • Sequence similarities
    Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
    Contains 7 C2H2-type zinc fingers.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ42816 antibody
    • HSPC082 antibody
    • KIAA0569 antibody
    • SIP 1 antibody
    • SIP1 antibody
    • Smad Interacting Protein 1 antibody
    • Smad-interacting protein 1 antibody
    • SMADIP 1 antibody
    • SMADIP1 antibody
    • ZEB 2 antibody
    • Zeb2 antibody
    • ZEB2_HUMAN antibody
    • Zfhx1b antibody
    • ZFHX1B protein antibody
    • Zfx1b antibody
    • Zinc finger E box binding protein 2 antibody
    • Zinc finger E-box-binding homeobox 2 antibody
    • Zinc finger homeobox 1b antibody
    • zinc finger homeobox protein 1 antibody
    • Zinc finger homeobox protein 1b antibody
    see all

Images

  • Flow cytometric analysis of MDA-MB435 cells (green, right histogram) labeling Smad Interacting Protein 1 using ab170759 at a 1/10 dilution, compared with negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-ZEB2 antibody - C-terminal (ab170759) at 1/100 dilution + MDA-MB435 cell lysate at 35 µg

    Predicted band size: 136 kDa

References

ab170759 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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