Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] (ab71286)

Overview

  • Product nameAnti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2]
    See all Zebrafish Gut Secretory Cell Epitopes primary antibodies
  • Description
    Mouse monoclonal [FIS 2F11/2] to Zebrafish Gut Secretory Cell Epitopes
  • SpecificityThe transparency of the juvenile zebrafish andits genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the secretory cells of the zebrafish intestinal epithelium, both mucous and enteroendocrine, corresponding to the class of cells that are lost in MathI mouse mutants (Yang et al, (2001) Science 294: 2155 2158). The antibody also labels hepatopancreatic ducts and intrahepatic bile ducts.
  • Tested applicationsSuitable for: ELISA, WB, IP, ICC/IF, IHC - Wholemount, ICCmore details
  • Species reactivity
    Reacts with: Zebrafish
  • Immunogen

    Tissue/ cell preparation (Zebrafish): Lysate of Zebrafish intestine.

Properties

Applications

Our Abpromise guarantee covers the use of ab71286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC - Wholemount 1/100. PubMed: 21385764
ICC Use at an assay dependent concentration.

Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] images

  • Immunohistochemical analysis of Zebrafish larvae, staining Zebrafish Gut Secretory Cell Epitopes with ab71286.

    Zebrafish larvae were fixed at 5 dpf in 4% paraformaldehyde before incubating with primary antibody (1/500) followed by a FITC-conjugated rabbit anti-mouse IgG (1/50) secondary antibody.

References for Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] (ab71286)

This product has been referenced in:
  • Tomé WA  et al. A mouse model replicating hippocampal sparing cranial irradiation in humans: A tool for identifying new strategies to limit neurocognitive decline. Sci Rep 5:14384 (2015). Read more (PubMed: 26399509) »
  • Ningappa M  et al. The Role of ARF6 in Biliary Atresia. PLoS One 10:e0138381 (2015). IHC ; Zebrafish . Read more (PubMed: 26379158) »

See all 13 Publications for this product

Product Wall

This antibody has cited to be used in whole mount immunohistochemistry in a number of publications e.g. Dev Biol 376:187-97 (2013), Dev Biol 372:178-89 (2012),   Development 138:1339-48 (2011), Development 138:3557-68 (2011). The protocol researchers h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"