Overview

  • Product name
    Anti-ZFAT antibody
  • Description
    Rabbit polyclonal to ZFAT
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 825- 1083 of Human ZFAT (Q9P243)

  • Positive control
    • Jurkat or Raji lysate, HBL435 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 139 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.

Target

  • Function
    May be involved in transcriptional regulation. Overexpression causes down-regulation of a number of genes involved in the immune response. Some genes are also up-regulated.
  • Tissue specificity
    Isoform 1 is strongly expressed in placenta, spleen, kidney, testis and peripheral blood leukocytes. Expressed in CD4+ and CD8+ T-cells, CD19+ B-cells and CB14+ monocytes. Isoform 3 is strongly expressed in placenta, ovary, tonsil, CD19+ B-cells and CD14+ monocytes.
  • Sequence similarities
    Contains 19 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • AITD3 antibody
    • KIAA1485 antibody
    • MGC126815 antibody
    • MGC126817 antibody
    • ZFAT antibody
    • ZFAT zinc finger 1 antibody
    • ZFAT_HUMAN antibody
    • ZFAT1 antibody
    • zinc finger and AT hook domain containing antibody
    • Zinc finger gene in AITD susceptibility region antibody
    • zinc finger gene in autoimmune thyroid disease antibody
    • Zinc finger protein 406 antibody
    • Zinc finger protein ZFAT antibody
    • ZNF406 antibody
    see all

Images

  • All lanes : Anti-ZFAT antibody (ab154722) at 1/1000 dilution

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 139 kDa



    5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human HBL435 xenograft tissue labeling ZFAT with ab151722 at a 1/500 dilution.

References

ab154722 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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