The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml. Note: ab68116 must be purified before use in ICC/IF.
1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
FunctionTranscriptional repressor of MYC and thymidine kinase promoters (By similarity). Transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Contains 1 BTB (POZ) domain. Contains 5 C2H2-type zinc fingers.
DomainThe BTB/POZ domain seems to direct the protein to discrete regions in the nucleus.