Overview

  • Product name
  • Description
    Mouse polyclonal to ZFP161
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    ZFP161 (NP_003400.2, 1 a.a. ~ 449 a.a) full-length human protein.

  • Positive control
    • HeLa cells, transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml. Note: ab68116 must be purified before use in ICC/IF.
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • Function
    Transcriptional repressor of MYC and thymidine kinase promoters (By similarity). Transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 BTB (POZ) domain.
    Contains 5 C2H2-type zinc fingers.
  • Domain
    The BTB/POZ domain seems to direct the protein to discrete regions in the nucleus.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HZF5 antibody
    • MGC126126 antibody
    • ZBTB14 antibody
    • ZF161_HUMAN antibody
    • ZF5 antibody
    • Zfp 161 antibody
    • Zfp-161 antibody
    • Zfp-5 antibody
    • Zfp161 antibody
    • Zinc finger and BTB domain containing protein 14 antibody
    • Zinc finger and BTB domain-containing protein 14 antibody
    • Zinc finger protein 161 homolog (mouse) antibody
    • Zinc finger protein 161 homolog antibody
    • Zinc finger protein 478 antibody
    • Zinc finger protein 5 antibody
    • Zinc finger protein 5 homolog antibody
    • Zinc finger protein homologous to Zfp161 in mouse antibody
    • ZNF478 antibody
    see all

Anti-ZFP161 antibody images

  • All lanes : Anti-ZFP161 antibody (ab68116) at 1/500 dilution

    Lane 1 : ZFP161 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • HeLa cells were labelled with purified ab68116 at 10 µg/ml followed by fluorescein conjugated secondary antibody. Cells were fixed using 4% paraformaldehyde and permeabilised with 0.1% Triton X-100

References for Anti-ZFP161 antibody (ab68116)

This product has been referenced in:
  • Maia AT  et al. Effects of BRCA2 cis-regulation in normal breast and cancer risk amongst BRCA2 mutation carriers. Breast Cancer Res 14:R63 (2012). ChIP . Read more (PubMed: 22513257) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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