Overview

  • Product nameAnti-Zfp219 antibody
    See all Zfp219 primary antibodies
  • Description
    Rabbit polyclonal to Zfp219
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Mouse Zfp219.

    (Peptide available as ab31002.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: F9; IOUD2.

Properties

Applications

Our Abpromise guarantee covers the use of ab31003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).

Target

Anti-Zfp219 antibody images

  • All lanes : Anti-Zfp219 antibody (ab31003) at 1/250 dilution

    Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg/ml per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 78 kDa
    Additional bands at : 58 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-Zfp219 antibody (ab31003)

ab31003 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount 50 µg
Specification Mouse ES Cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Utkarsh Kapoor

Verified customer

Submitted Dec 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Embryonic Stem Cells & Embryoid Bodies)
Loading amount 40 µg
Specification Mouse Embryonic Stem Cells & Embryoid Bodies
Gel Running Conditions Reduced Denaturing (12% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Utkarsh Kapoor

Verified customer

Submitted Aug 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"