Anti-Zfp219 antibody - Aminoterminal end (ab71279)

Overview

  • Product nameAnti-Zfp219 antibody - Aminoterminal end
    See all Zfp219 primary antibodies
  • Description
    Rabbit polyclonal to Zfp219 - Aminoterminal end
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to N terminal amino acids 161-191 of Human Zfp219

  • Positive control
    • 293 cell line lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab71279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 30, 55, 60, 77 kDa (predicted molecular weight: 77 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

Anti-Zfp219 antibody - Aminoterminal end images

  • Anti-Zfp219 antibody - Aminoterminal end (ab71279) at 1/60 dilution + 293 cell line lysates at 35 µg

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 30 kDa,55 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • ab71279 at 1/50 dilution staining Zfp219 in human testis tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References for Anti-Zfp219 antibody - Aminoterminal end (ab71279)

This product has been referenced in:
  • Takigawa Y  et al. The transcription factor Znf219 regulates chondrocyte differentiation by assembling a transcription factory with Sox9. J Cell Sci 123:3780-8 (2010). IHC-P ; Mouse . Read more (PubMed: 20940257) »

See 1 Publication for this product

Product Wall

I understand that you have a deadline coming up quickly and I really wish I could hurry the production process along. We do make every effort to produce the antibodies as quickly as possible, as you can imagine we can't sell the antibodies if they are ...

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Thanks for sending along the western blot and IHC data. That was very helpful. I agree it is odd that the same 2 intense bands are consistantly detected in all your blots. As well, the IHC staining should be largely nuclear and it is not. And, as you s...

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