Anti-Zfp281/ZNF281 antibody (ab101318)
Key features and details
- Rabbit polyclonal to Zfp281/ZNF281
- Suitable for: WB, IP
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Zfp281/ZNF281 antibody -
Description
Rabbit polyclonal to Zfp281/ZNF281 -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Rabbit, Horse, Dog, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Human Zfp281/ZNF281 aa 800 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab130905) -
Positive control
- This antibody gave a positive signal in a wild type mouse embryonic stem cell lysate, which is absent from a Zfp281/ZNF281 null mouse embryonic stem cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
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IP | (1) |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23528 Human
- Entrez Gene: 226442 Mouse
- Entrez Gene: 305083 Rat
- SwissProt: Q9Y2X9 Human
- Unigene: 59757 Human
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Alternative names
- GC box binding zinc finger protein 1 antibody
- GC-box-binding zinc finger protein 1 antibody
- GZP1 antibody
see all
Images
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All lanes : Anti-Zfp281/ZNF281 antibody (ab101318) at 1 µg/ml
Lane 1 : wild type mouse embryonic stem cell lysate
Lane 2 : Zfp281/ZNF281 null mouse embryonic stem cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab101318 has been referenced in 10 publications.
- LeBlanc L et al. β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiation. iScience 25:103541 (2022). PubMed: 34977504
- Laudadio I et al. ZNF281 Promotes Colon Fibroblast Activation in TGFβ1-Induced Gut Fibrosis. Int J Mol Sci 23:N/A (2022). PubMed: 36142169
- Nicolai S et al. ZNF281/Zfp281 is a target of miR-1 and counteracts muscle differentiation. Mol Oncol 14:294-308 (2020). PubMed: 31782884
- Xian Z et al. lncRNA UCA1 Contributes to 5-Fluorouracil Resistance of Colorectal Cancer Cells Through miR-23b-3p/ZNF281 Axis. Onco Targets Ther 13:7571-7583 (2020). PubMed: 32801774
- Guo J et al. The transcription factor Zfp281 sustains CD4+ T lymphocyte activation through directly repressing Ctla-4 transcription. Cell Mol Immunol N/A:N/A (2019). PubMed: 31511645
- Ishiuchi T et al. Zfp281 Shapes the Transcriptome of Trophoblast Stem Cells and Is Essential for Placental Development. Cell Rep 27:1742-1754.e6 (2019). PubMed: 31067460
- Huang X et al. Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling. Elife 6:N/A (2017). PubMed: 29168693
- Fidalgo M et al. Zfp281 Coordinates Opposing Functions of Tet1 and Tet2 in Pluripotent States. Cell Stem Cell 19:355-69 (2016). PubMed: 27345836
- Pieraccioli M et al. ZNF281 contributes to the DNA damage response by controlling the expression of XRCC2 and XRCC4. Oncogene 35:2592-601 (2016). PubMed: 26300006
- Fidalgo M et al. Zfp281 mediates Nanog autorepression through recruitment of the NuRD complex and inhibits somatic cell reprogramming. Proc Natl Acad Sci U S A 109:16202-7 (2012). WB, ChIP, IP . PubMed: 22988117