Overview

  • Product nameAnti-Zfp281 antibody
    See all Zfp281 primary antibodies
  • Description
    Rabbit polyclonal to Zfp281
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Dog, Human, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human Zfp281.

  • Positive control
    • This antibody gave a positive signal in a wild type mouse embryonic stem cell lysate, which is absent from a Zfp281 null mouse embryonic stem cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab101318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
IP Use at an assay dependent concentration.

Target

  • FunctionInvolved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GC box binding zinc finger protein 1 antibody
    • GC-box-binding zinc finger protein 1 antibody
    • GZP1 antibody
    • Transcription factor ZBP-99 antibody
    • ZBP 99 antibody
    • ZBP99 antibody
    • zfp281 antibody
    • Zinc finger DNA binding protein 99 antibody
    • Zinc finger DNA-binding protein 99 antibody
    • Zinc finger protein 281 antibody
    • ZN281_HUMAN antibody
    • ZNF281 antibody
    • ZNP 99 antibody
    • ZNP 99 transcription factor antibody
    • ZNP99 antibody
    see all

Anti-Zfp281 antibody images

  • All lanes : Anti-Zfp281 antibody (ab101318) at 1 µg/ml

    Lane 1 : wild type mouse embryonic stem cell lysate
    Lane 2 : Zfp281 null mouse embryonic stem cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 97 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • Zfp281 was immunoprecipitated using 2µg/ml of ab101318 and 2mg of wild type Mouse embryonic stem cell nuclear extract (lane 1). Mouse embryonic stem cells negative for Zfp281 were used as a negative control (lane 2). Band at ~98kDa is Zfp281.

    See Abreview

References for Anti-Zfp281 antibody (ab101318)

This product has been referenced in:
  • Fidalgo M  et al. Zfp281 mediates Nanog autorepression through recruitment of the NuRD complex and inhibits somatic cell reprogramming. Proc Natl Acad Sci U S A 109:16202-7 (2012). WB, ChIP, IP . Read more (PubMed: 22988117) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - nuclear (mouse embryonic stem cells)
Total protein in input 2000 µg
Specification mouse embryonic stem cells
Immuno-precipitation step Protein G
Username

Dr. Miguel Fidalgo

Verified customer

Submitted May 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (mouse embryonic stem cells)
Loading amount 30 µg
Specification mouse embryonic stem cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Miguel Fidalgo

Verified customer

Submitted May 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"