This antibody gave a positive signal in a wild type mouse embryonic stem cell lysate, which is absent from a Zfp281 null mouse embryonic stem cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
Use at an assay dependent concentration.
FunctionInvolved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Contains 4 C2H2-type zinc fingers.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Immunoprecipitation - Anti-Zfp281 antibody (ab101318)This image is courtesy of an abreview by Miguel Fidalgo.
Zfp281 was immunoprecipitated using 2µg/ml of ab101318 and 2mg of wild type Mouse embryonic stem cell nuclear extract (lane 1). Mouse embryonic stem cells negative for Zfp281 were used as a negative control (lane 2). Band at ~98kDa is Zfp281.
Fidalgo M et al. Zfp281 mediates Nanog autorepression through recruitment of the NuRD complex and inhibits somatic cell reprogramming. Proc Natl Acad Sci U S A109:16202-7 (2012).
WB, ChIP, IP
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