FunctionInvolved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Contains 4 C2H2-type zinc fingers.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
All lanes : Anti-Zfp281 antibody (ab112047) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 97 kDa
Exposure time : 3 minutes
Immunoprecipitation - Zfp281 antibody (ab112047)
Detection of Zfp281 by Western Blot of Immunprecipitate.
ab112047 at 1µg/ml staining Zfp281 in HeLa whole cell lysate immunoprecipitated using ab112047 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.