The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay, 1:312500.
FunctionInvolved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Contains 4 C2H2-type zinc fingers.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.