Overview

  • Product nameAnti-Zfp281 antibody
    See all Zfp281 primary antibodies
  • Description
    Mouse monoclonal to Zfp281
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment: KLTSQKEQKN LESSTGFQIP SQELASQIDP QKDIEPRTTY QIENFAQAFG , corresponding to amino acids 787-894 of Human Zfp281

  • Positive control
    • A431 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab89445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionInvolved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GC box binding zinc finger protein 1 antibody
    • GC-box-binding zinc finger protein 1 antibody
    • GZP1 antibody
    • Transcription factor ZBP-99 antibody
    • ZBP 99 antibody
    • ZBP99 antibody
    • zfp281 antibody
    • Zinc finger DNA binding protein 99 antibody
    • Zinc finger DNA-binding protein 99 antibody
    • Zinc finger protein 281 antibody
    • ZN281_HUMAN antibody
    • ZNF281 antibody
    • ZNP 99 antibody
    • ZNP 99 transcription factor antibody
    • ZNP99 antibody
    see all

Anti-Zfp281 antibody images

  • Anti-Zfp281 antibody (ab89445) at 1 µg/ml + A431 cell lysate at 50 µg

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • Anti-Zfp281 antibody (ab89445) at 1 µg/ml + recombinant immunogen at 0.2 µg

    Predicted band size : 97 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-Zfp281 antibody (ab89445)

ab89445 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (embryonic stem cells)
Loading amount 40 µg
Specification embryonic stem cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jul 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"