• Product nameAnti-Zfp57 antibody
    See all Zfp57 primary antibodies
  • Description
    Rabbit polyclonal to Zfp57
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse Zfp57.

    (Peptide available as ab45340.)

  • Positive control
    • This antibody also gave a positive signal in the following Mouse Whole Cell Lysate: NIH 3T3 (ab7179) (data not shown)



Our Abpromise guarantee covers the use of ab45341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 48 kDa).Can be blocked with Mouse Zfp57 peptide (ab45340).


  • RelevanceZfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • bA145L22 antibody
    • bA145L22.2 antibody
    • C6orf40 antibody
    • TNDM1 antibody
    • Zfp 57 antibody
    • ZFP57 zinc finger protein antibody
    • Zinc Finger Protein 57 antibody
    • Zinc finger protein 57 homolog antibody
    • Zinc finger protein 698 antibody
    • ZNF698 antibody
    see all

Anti-Zfp57 antibody images

  • Anti-Zfp57 antibody (ab45341) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate (ab50253) at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)

    Exposure time : 150 seconds

References for Anti-Zfp57 antibody (ab45341)

This product has been referenced in:
  • Riso V  et al. ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells. Nucleic Acids Res 44:8165-78 (2016). Read more (PubMed: 27257070) »
  • Baglivo I  et al. Genetic and epigenetic mutations affect the DNA binding capability of human ZFP57 in transient neonatal diabetes type 1. FEBS Lett 587:1474-81 (2013). ChIP ; Mouse . Read more (PubMed: 23499433) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us. No, these antibodies have not been tested with overexpression or knockdown cells.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

Our testing did show a band at this range in mouse brain lysate as well (http://www.abcam.com/Zfp57-antibody-ab45341.html#ab45341_1.jpg) that we were not able to identify. To our knowledge, this product has not...

Read More

Thank you for contacting us. Unfortunately it is unlikely that ab45341 works in Human (poor sequence homology). Thank you for sending us the reference of the publication in which ab45341 was used. About ab50944, you mentioned that another lab ...

Read More
Application Immunoprecipitation
Sample Mouse Tissue lysate - whole (Testes)
Total protein in input 20 µg
Specification Testes
Immuno-precipitation step Protein G

Abcam user community

Verified customer

Submitted Aug 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Uterus)
Specification Uterus
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%

Abcam user community

Verified customer

Submitted Apr 01 2008