Overview

  • Product name
  • Description
    Rabbit polyclonal to Zfp57
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ChIP, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse Zfp57.

    (Peptide available as ab45340.)

  • Positive control
    • This antibody also gave a positive signal in the following Mouse Whole Cell Lysate: NIH 3T3 (ab7179) (data not shown)

Properties

Applications

Our Abpromise guarantee covers the use of ab45341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 26728555
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 48 kDa).Can be blocked with Mouse Zfp57 peptide (ab45340).

Target

  • Relevance
    Zfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • bA145L22 antibody
    • bA145L22.2 antibody
    • C6orf40 antibody
    • TNDM1 antibody
    • Zfp 57 antibody
    • ZFP57 zinc finger protein antibody
    • Zinc Finger Protein 57 antibody
    • Zinc finger protein 57 homolog antibody
    • Zinc finger protein 698 antibody
    • ZNF698 antibody
    see all

Images

  • Anti-Zfp57 antibody (ab45341) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate (ab50253) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 57 kDa (why is the actual band size different from the predicted?)


    Exposure time: 150 seconds

References

This product has been referenced in:
  • Luo Z  et al. Regulation of the imprinted Dlk1-Dio3 locus by allele-specific enhancer activity. Genes Dev 30:92-101 (2016). CHIPseq ; Mouse . Read more (PubMed: 26728555) »
  • Riso V  et al. ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells. Nucleic Acids Res 44:8165-78 (2016). Read more (PubMed: 27257070) »

See all 6 Publications for this product

Customer reviews and Q&As

Filter by Application

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Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (Testes)
Total protein in input
20 µg
Specification
Testes
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

Submitted Aug 02 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Uterus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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