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Rabbit polyclonal to Zfp57
Predicted to work with:
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse Zfp57.
(Peptide available as
This antibody also gave a positive signal in the following Mouse Whole Cell Lysate: NIH 3T3 (
ab7179) (data not shown)
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 26728555
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 48 kDa).Can be blocked with
Mouse Zfp57 peptide (ab45340).
Zfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF.
Western blot - Anti-Zfp57 antibody (ab45341)
Anti-Zfp57 antibody (ab45341) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate (
) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) (
) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
57 kDa (
why is the actual band size different from the predicted?
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