Anti-Zfp57 antibody (ab50944)

Overview

  • Product nameAnti-Zfp57 antibody
    See all Zfp57 primary antibodies
  • Description
    Rabbit polyclonal to Zfp57
  • SpecificityThis antibody reacts with Zfp57
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: MFEQLKPIEP RDCWREARVK KKPVTFEDVA VNFTQEEWDC LDASQRVLYQ, corresponding to N terminal amino acids 1-50 of Human Zfp57

  • Positive control
    • WB: Jurkat cell lysate IHC-P: Human kidney tissue IF/ICC: MEF1 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab50944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • RelevanceZfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • bA145L22 antibody
    • bA145L22.2 antibody
    • C6orf40 antibody
    • TNDM1 antibody
    • Zfp 57 antibody
    • ZFP57 zinc finger protein antibody
    • Zinc Finger Protein 57 antibody
    • Zinc finger protein 57 homolog antibody
    • Zinc finger protein 698 antibody
    • ZNF698 antibody
    see all

Anti-Zfp57 antibody images

  • ICC/IF image of ab50944 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50944, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Zfp57 antibody (ab50944) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000

    Predicted band size : 59 kDa
    Observed band size : 64,65,66 kDa (why is the actual band size different from the predicted?)
  • Ab50944, at a concentration of 4µg/ml, staining Zpf57 in the epithelial cells of human kidney renal tubules. Magnification: x400.Ab50944, at a concentration of 4µg/ml, staining Zpf57 in the epithelial cells of human kidney renal tubules. Magnification: x400.

References for Anti-Zfp57 antibody (ab50944)

ab50944 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Specification kidney
Blocking step Protein Block (Leica) as blocking agent for 5 minute(s) · Concentration: 0.4% · Temperature: 21°C
Fixative Formaldehyde
Username

Ms. Eva Rodriguez Nunez

Verified customer

Submitted Aug 05 2015

Thank you for contacting us. Unfortunately it is unlikely that ab45341 works in Human (poor sequence homology). Thank you for sending us the reference of the publication in which ab45341 was used. About ab50944, you mentioned that another lab ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"