Anti-Zfp57 antibody (ab50944)

Overview

  • Product name
  • Description
    Rabbit polyclonal to Zfp57
  • Host species
    Rabbit
  • Specificity
    This antibody reacts with Zfp57
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: MFEQLKPIEP RDCWREARVK KKPVTFEDVA VNFTQEEWDC LDASQRVLYQ, corresponding to N terminal amino acids 1-50 of Human Zfp57

  • Positive control
    • WB: Jurkat cell lysate IHC-P: Human kidney tissue IF/ICC: MEF1 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab50944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance
    Zfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • bA145L22 antibody
    • bA145L22.2 antibody
    • C6orf40 antibody
    • TNDM1 antibody
    • Zfp 57 antibody
    • ZFP57 zinc finger protein antibody
    • Zinc Finger Protein 57 antibody
    • Zinc finger protein 57 homolog antibody
    • Zinc finger protein 698 antibody
    • ZNF698 antibody
    see all

Images

  • ICC/IF image of ab50944 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50944, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Zfp57 antibody (ab50944) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000

    Predicted band size: 59 kDa
    Observed band size: 64,65,66 kDa (why is the actual band size different from the predicted?)

  • Ab50944, at a concentration of 4µg/ml, staining Zpf57 in the epithelial cells of human kidney renal tubules. Magnification: x400.

    Ab50944, at a concentration of 4µg/ml, staining Zpf57 in the epithelial cells of human kidney renal tubules. Magnification: x400.

References

ab50944 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Specification
kidney
Blocking step
Protein Block (Leica) as blocking agent for 5 minute(s) · Concentration: 0.4% · Temperature: 21°C
Fixative
Formaldehyde
Username

Ms. Eva Rodriguez Nunez

Verified customer

Submitted Aug 05 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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