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Rabbit polyclonal to ZFP64
Predicted to work with:
Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 400-450 of Human ZFP64 (NP_060667.2).
Jurkat, HeLa, 293T and NIH 3T3 whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
Immunogen affinity purified
ab117787 was affinity purified using an epitope specific to ZFP64 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 74 kDa.
Use at 2-10 µg/mg of lysate.
ZFP64 belongs to the krueppel C
2H 2-type zinc-finger protein family. It contains 9 C 2H 2-type zinc fingers and may be involved in transcriptional regulation.
Western blot - Anti-ZFP64 antibody (ab117787)
All lanes : Anti-ZFP64 antibody (ab117787) at 0.04 µg/ml Lane 1 : 293T whole cell lysates at 50 µg Lane 2 : 293T whole cell lysates at 15 µg Lane 3 : Jurkat whole cell lysates at 50 µg Lane 4 : HeLa whole cell lysates at 50 µg Lane 5 : NIH 3T3 whole cell lysates at 50 µg Developed using the ECL technique Predicted band size : 74 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-ZFP64 antibody (ab117787)
Detection of ZFP64 by Western Blot of Immunprecipitate.
ab117787 at 1µg/ml staining ZFP64 in 293T whole cell lysate immunoprecipitated using ab117787 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"