The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
The protein encoded by ZFP91 is a member of the zinc finger family of proteins. This protein contains C2H2 type domains, which are the classical zinc finger domains found in numerous nucleic acid-binding proteins.