Overview

  • Product name
  • Description
    Rabbit polyclonal to ZFPL1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide derived from a region within residues 101-150 of human ZFPL1.

    .

  • Positive control
    • HepG2 cell lysate for Western blot and human kidney tissue for Immunohistochemistry.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None.
    Constituents: 2% Sucrose, PBS.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    ZFPL1 is a regulatory transcription factor that is expressed strongly in the exocrine pancreas. It contains 1 RING type zinc finger. It is required for cis-Golgi integrity and efficient ER to Golgi transport. Involved in the maintainance of the integrity of the cis-Golgi, possibly via its interaction with GOLGA2/GM130.
  • Cellular localization
    Golgi Apparatus and Nuclear; Single-pass membrane protein.
  • Database links
  • Alternative names
    • D11S750 antibody
    • MCG4 antibody
    • MGC 4 antibody
    • ZFPL 1 antibody
    • Zinc finger protein like 1 antibody
    • Zinc finger protein MCG4 antibody
    • zinc-finger protein in MEN1 region antibody
    see all

Images

  • Anti-ZFPL1 antibody (ab26057) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate 10ug.

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Ab26057 at 4-8 ug/ml staining ZFPL1 in paraffin embedded human kidney tissue by Immunohistochemistry. Arrows indicate positively labelled epithelial cells of the renal tube.

References

ab26057 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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