The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ZFYVE1 contains two zinc-binding FYVE domains in tandem. The FYVE domain mediates the recruitment of proteins involved in membrane trafficking and cell signaling to phosphatidylinositol 3-phosphate (PtdIns(3)P)-containing membranes. There are two named isoforms of ZFYVE1.
Golgi apparatus, Golgi stack.
Note: Resides predominantly in the cisternal stacks of the Golgi.