Overview

  • Product nameAnti-ZFYVE1 antibody
    See all ZFYVE1 primary antibodies
  • Description
    Rabbit polyclonal to ZFYVE1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (VPHEAKSRCR YSHQYDNRVY TCKACYERGE EVSVVPKTSA STDSPWMGLA) of Human ZFYVE1 (NP_848535).

  • Positive control
    • Fetal stomach lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceZFYVE1 contains two zinc-binding FYVE domains in tandem. The FYVE domain mediates the recruitment of proteins involved in membrane trafficking and cell signaling to phosphatidylinositol 3-phosphate (PtdIns(3)P)-containing membranes. There are two named isoforms of ZFYVE1.
  • Cellular localizationGolgi apparatus, Golgi stack. Note: Resides predominantly in the cisternal stacks of the Golgi.
  • Database links
  • Alternative names
    • DFCP1 antibody
    • Double FYVE containing protein 1 antibody
    • KIAA1589 antibody
    • Phosphoinositide binding protein SR3 antibody
    • SR3 antibody
    • TAFF1 antibody
    • Tandem FYVE fingers 1 antibody
    • ZFYVE 1 antibody
    • Zinc finger FYVE domain containing protein 1 antibody
    • Zinc finger protein subfamily 2A (FYVE domain containing) 1 antibody
    • Zinc finger protein subfamily 2A member 1 antibody
    • ZNFN2A1 antibody
    see all

Anti-ZFYVE1 antibody images

  • Anti-ZFYVE1 antibody (ab90029) at 1 µg/ml (5% skim milk / PBS buffer) + Human fetal stomach lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa

References for Anti-ZFYVE1 antibody (ab90029)

ab90029 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Zebrafish Tissue lysate - whole (whole embryos)
Specification whole embryos
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Evgenia Salta

Verified customer

Submitted Feb 05 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"