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Storage instructions Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage buffer Preservative: 0.09% Sodium Azide
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab103683 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: for isoform 2, 88 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Function May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes.
Tissue specificity Widely expressed. Highly expressed in kidney, placenta and lung. Expressed at intermediate level in heart, brain, skeletal muscle, spleen and liver. Weakly expressed in colon, thymus and peripheral blood lymphocytes.
Sequence similarities Contains 1 FYVE-type zinc finger.
Domain The FYVE-type zinc finger is necessary and sufficient for its localization into early endosomes and mediates the association with PI3P.
Cellular localization Cytoplasm. Early endosome membrane. Localized to early endosomes. Membrane-associated, probably via its association with phosphatidylinositol 3-phosphate.
Information by UniProt
Anti-ZFYVE16 antibody images
Western blot - ZFYVE16 antibody - N-terminal (ab103683)
Anti-ZFYVE16 antibody (ab103683) at 1/100 dilution + K562 cell line lysates at 35 µg Predicted band size : for isoform 2, 88 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ZFYVE16 antibody - N-terminal (ab103683)
ab103683, at a 1/50 dilution, staining ZFYVE16 in formalin fixed, paraffin embedded Human lung tissue by Immunohistochemistry, followed by peroxidase-conjugated secondary antibody and DAB staining.
References for Anti-ZFYVE16 antibody (ab103683)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"