Overview

  • Product nameAnti-ZFYVE27 antibody
    See all ZFYVE27 primary antibodies
  • Description
    Rabbit polyclonal to ZFYVE27
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to an internal sequence within amino acids 253-302 of Human ZFYVE27 GKDGLMDSTP ALTPTESLSS QDLTPGSVEE AEEAEPDEEF KDAIEETHLV

  • Positive control
    • Jurkat membrane lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab61285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionFunctions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Involved in nerve growth factor-induced neurite formation. May have a more general role in cell projections formation.
  • Involvement in diseaseDefects in ZFYVE27 are the cause of spastic paraplegia autosomal dominant type 33 (SPG33) [MIM:610244]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Note=According to PubMed:18606302, the properties of the variant Val-191 and its frequency in some populations raise doubts on the implication of that gene in the disease.
  • Sequence similaritiesContains 1 FYVE-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation is induced by NGF through the MAPK/ERK pathway and modulates interaction with RAB11A.
  • Cellular localizationRecycling endosome membrane. Endoplasmic reticulum membrane. Cell projection > growth cone membrane. Cell membrane. Localizes at both dendrites and axons.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protrudin antibody
    • RP11 459F3.2 antibody
    • SPG33 antibody
    • ZFY27_HUMAN antibody
    • ZFYVE27 antibody
    • zinc finger FYVE domain containing 27 antibody
    • Zinc finger FYVE domain containing protein 27 antibody
    • Zinc finger FYVE domain-containing protein 27 antibody
    see all

Anti-ZFYVE27 antibody images

  • Anti-ZFYVE27 antibody (ab61285) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-ZFYVE27 antibody (ab61285)

ab61285 has not yet been referenced specifically in any publications.

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