The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 105 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein in western blotting.
Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Place sample in 1X citrate buffer (pH 6.0) in pressure cooker under 125oC for 4min and under 90oC for 45min.
FunctionActs as a transcriptional repressor. Is a regulator of podocyte gene expression during primary glomerula disease.
Tissue specificityWidely expressed. High expression in kidney.
Sequence similaritiesBelongs to the ZHX family. Contains 2 C2H2-type zinc fingers. Contains 5 homeobox DNA-binding domains.