Overview

  • Product nameAnti-ZHX3 antibody
    See all ZHX3 primary antibodies
  • Description
    Rabbit polyclonal to ZHX3
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 150-200 of Human ZHX3 (NP_055850.1)

  • Positive control
    • HeLa and 293T whole cell lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab99350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
IP Use a concentration of 2 - 5 µg/ml.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionActs as a transcriptional repressor. Is a regulator of podocyte gene expression during primary glomerula disease.
  • Tissue specificityWidely expressed. High expression in kidney.
  • Sequence similaritiesBelongs to the ZHX family.
    Contains 2 C2H2-type zinc fingers.
    Contains 5 homeobox DNA-binding domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0395 antibody
    • TIX1 antibody
    • Triple homeobox 1 antibody
    • Triple homeobox protein 1 antibody
    • ZHX 3 antibody
    • Zhx3 antibody
    • ZHX3_HUMAN antibody
    • Zinc finger and homeodomain protein 3 antibody
    • Zinc fingers and homeoboxes protein 3 antibody
    see all

Anti-ZHX3 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZHX3 with ab99350 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-ZHX3 antibody (ab99350) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 105 kDa


    Exposure time : 30 seconds
  • Detection of Human ZHX3 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells. ab99350 used for WB at 1 ug/ml and used for IP at 3 ug/mg lysate (lane 1). ZHX3 was also immunoprecipitated by another rabbit anti-ZHX3 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 10 seconds.

References for Anti-ZHX3 antibody (ab99350)

ab99350 has not yet been referenced specifically in any publications.

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