Anti-Zic2 antibody (ab10550)

Overview

• Product nameAnti-Zic2 antibody
  See all Zic2 primary antibodies
• Description
  Rabbit polyclonal to Zic2
• SpecificityRecognises a band at about 60kD in mouse brain lysate (but not in rat brain lysate). A lower band is also detected, the identity of this is unknown.
• Tested applicationsSuitable for: WBmore details
• Species reactivity
  Reacts with: Mouse
  Predicted to work with: Cow, Human, Zebrafish
• Immunogen

  Synthetic peptide:

  GRSEQYRQVASPRTDPYS

  conjugated to KLH, corresponding to amino acids 190-207 of Mouse Zic2.
  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
• Concentration information loading...
• PurityImmunogen affinity purified
• ClonalityPolyclonal
• IsotypeIgG
• Research areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• FunctionActs as a transcriptional activator or repressor. Plays important roles in the early stage of organogenesis of the CNS. Activates the transcription of the serotonin transporter SERT in uncrossed ipsilateral retinal ganglion cells (iRGCs) to refine eye-specific projections in primary visual targets. Its transcriptional activity is repressed by MDFIC. Involved in the formation of the ipsilateral retinal projection at the optic chiasm midline. Drives the expression of EPHB1 on ipsilaterally projecting growth cones. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'. Associates to the basal SERT promoter region from ventrotemporal retinal segments of retinal embryos.
• Involvement in diseaseDefects in ZIC2 are a cause of holoprosencephaly type 5 (HPE5) [MIM:609637]. A structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. Although severe facial anomalies are common in HPE, patients with ZINC2 mutations have relatively normal faces.
• Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
  Contains 5 C2H2-type zinc fingers.
• DomainThe C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.
• Post-translational
  modificationsPhosphorylated.
  Ubiquitinated by RNF180, leading to its degradation.
• Cellular localizationNucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression. Both phosphorylated and unphosphorylated forms are localized in the nucleus.

• Target information above from: UniProt accession O95409 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 514433 Cow
  • Entrez Gene: 7546 Human
  • Entrez Gene: 22772 Mouse
  • Omim: 603073 Human
  • SwissProt: O95409 Human
  • SwissProt: Q62520 Mouse
  • Unigene: 653700 Human
  • Unigene: 308936 Mouse
• Alternative names
  • HPE 5 antibody
  • HPE5 antibody
  • Odd paired homolog Drosophila antibody

  • Zic 2 antibody
  • Zic family member 2 (odd paired Drosophila homolog) antibody
  • Zic family member 2 antibody
  • ZIC2 antibody
  • ZIC2_HUMAN antibody
  • Zinc finger protein of the cerebellum 2 antibody
  • Zinc finger protein ZIC 2 antibody
  • Zinc finger protein Zic2 antibody

  see all