Anti-Zic2 antibody (ab10550)


  • Product nameAnti-Zic2 antibody
    See all Zic2 primary antibodies
  • Description
    Rabbit polyclonal to Zic2
  • SpecificityRecognises a band at about 60kD in mouse brain lysate (but not in rat brain lysate). A lower band is also detected, the identity of this is unknown.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Cow, Human, Zebrafish
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 190-207 of Mouse Zic2.



Our Abpromise guarantee covers the use of ab10550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).


  • FunctionActs as a transcriptional activator or repressor. Plays important roles in the early stage of organogenesis of the CNS. Activates the transcription of the serotonin transporter SERT in uncrossed ipsilateral retinal ganglion cells (iRGCs) to refine eye-specific projections in primary visual targets. Its transcriptional activity is repressed by MDFIC. Involved in the formation of the ipsilateral retinal projection at the optic chiasm midline. Drives the expression of EPHB1 on ipsilaterally projecting growth cones. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'. Associates to the basal SERT promoter region from ventrotemporal retinal segments of retinal embryos.
  • Involvement in diseaseDefects in ZIC2 are a cause of holoprosencephaly type 5 (HPE5) [MIM:609637]. A structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. Although severe facial anomalies are common in HPE, patients with ZINC2 mutations have relatively normal faces.
  • Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • DomainThe C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.
  • Post-translational
    Ubiquitinated by RNF180, leading to its degradation.
  • Cellular localizationNucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression. Both phosphorylated and unphosphorylated forms are localized in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HPE 5 antibody
    • HPE5 antibody
    • Odd paired homolog Drosophila antibody
    • Zic 2 antibody
    • Zic family member 2 (odd paired Drosophila homolog) antibody
    • Zic family member 2 antibody
    • ZIC2 antibody
    • ZIC2_HUMAN antibody
    • Zinc finger protein of the cerebellum 2 antibody
    • Zinc finger protein ZIC 2 antibody
    • Zinc finger protein Zic2 antibody
    see all

Anti-Zic2 antibody images

  • Predicted band size : 55 kDa

    Western blot using ab10550 at 1/500.

    Lane 1 : Mouse Brain tissue lysate
    Lane 2 : Rat Brain tissue lysate

    The upper band in the mouse brain lysate represents Zic2.

    Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
    Exposure time: 5 minutes
    Expected molecular weight: 55 kDa
    20µg of cell lysate per lane

References for Anti-Zic2 antibody (ab10550)

ab10550 has not yet been referenced specifically in any publications.

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As you have noticed, the Anti-Zic2 antibody (ab10550) has as yet not been tested in IP. This does not necessarily mean that it would not work, simply that we have not tested it and would not be able to guarantee i...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse forebrain E17)
Specification Mouse forebrain E17
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization Yes - 0.025% TX100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C

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Submitted Aug 21 2008