Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA).
Use a concentration of 0.5 - 2 µg/ml. In transfected RWPE1 transiently expressing Human ZIC2-Myc a band of approx. 65kDa is observed. This band is not observed in the non-transfected RWPE1, nor when GFP is transiently expressed. The calculated molecular size is 55.0kDa according to NP_009060.2 and 58.6kDa for the ZIC2-Myc protein. We have observed an approx 75kDa band in Human Brain (Cerebellum and Cerebral Cortex) lysates .
Acts as a transcriptional activator or repressor. Plays important roles in the early stage of organogenesis of the CNS. Activates the transcription of the serotonin transporter SERT in uncrossed ipsilateral retinal ganglion cells (iRGCs) to refine eye-specific projections in primary visual targets. Its transcriptional activity is repressed by MDFIC. Involved in the formation of the ipsilateral retinal projection at the optic chiasm midline. Drives the expression of EPHB1 on ipsilaterally projecting growth cones. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'. Associates to the basal SERT promoter region from ventrotemporal retinal segments of retinal embryos.
Involvement in disease
Defects in ZIC2 are a cause of holoprosencephaly type 5 (HPE5) [MIM:609637]. A structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. Although severe facial anomalies are common in HPE, patients with ZINC2 mutations have relatively normal faces.
Belongs to the GLI C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers.
The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.
Phosphorylated. Ubiquitinated by RNF180, leading to its degradation.
Nucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression. Both phosphorylated and unphosphorylated forms are localized in the nucleus.
Zic family member 2 (odd paired Drosophila homolog) antibody
Zic family member 2 antibody
Zinc finger protein of the cerebellum 2 antibody
Zinc finger protein ZIC 2 antibody
Zinc finger protein Zic2 antibody
Anti-Zic2 antibody images
This Fast-Track antibody is not yet fully characterised. These images represent
inconclusive preliminary data.
Western blot - Anti-Zic2 antibody (ab121036)
RWPE1 lysate (60ug protein in RIPA buffer) overexpressing Human ZIC2 with C-terminal MYC tag probed with ab121036 (0.5ug/ml) in the right panel(B) and probed with anti-MYC Tag (1/1000) and anti-beta-Actin in the left panel (A). Mock-transfected RWPE1 in lanes 1 and expressing GFP in lanes 2. Primary incubations were overnight at 4C. Detected by chemiluminescence.