Overview

  • Product name
  • Description
    Goat polyclonal to ZIC3
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Monkey, Opossum, Common marmoset, Orangutan, Bat, Platypus, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human ZIC3 aa 178-189 (internal sequence) (Cysteine residue).
    Sequence:

    HVDNNQVHLGLR


    Database link: O60481

  • Positive control
    • Human Prostate tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab189969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
  • Involvement in disease
    Heterotaxy, visceral, 1, X-linked
    VACTERL association X-linked with or without hydrocephalus
    Congenital heart defects, multiple types, 1, X-linked
  • Sequence similarities
    Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Domain
    The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.
  • Cellular localization
    Nucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression (By similarity). Translocation to the nucleus requires KPNA1 or KPNA6.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heterotaxy 1 antibody
    • HTX antibody
    • HTX1 antibody
    • VACTERLX antibody
    • ZIC 3 antibody
    • Zic family member 3 (odd paired Drosophila homolog heterotaxy 1) antibody
    • Zic family member 3 antibody
    • Zic3 antibody
    • ZIC3_HUMAN antibody
    • Zinc finger protein 203 antibody
    • Zinc finger protein of the cerebellum 3 antibody
    • Zinc finger protein ZIC 3 (Zinc finger protein of the cerebellum 3) antibody
    • Zinc finger protein ZIC 3 antibody
    • ZNF203 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human Prostate tissue labeling ZIC3 with ab189969 at 5 µg/ml.

References

ab189969 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Differentiated human embryonic stem cells)
Permeabilization
Yes - PBS/0.1% Triton X
Specification
Differentiated human embryonic stem cells
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
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Submitted Dec 23 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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