Anti-ZIC3 antibody (ab189969)
Key features and details
- Goat polyclonal to ZIC3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ZIC3 antibody
See all ZIC3 primary antibodies -
Description
Goat polyclonal to ZIC3 -
Host species
Goat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Monkey, Opossum, Common marmoset, Orangutan, Bat, Platypus, Elephant -
Immunogen
Synthetic peptide corresponding to Human ZIC3 aa 150-250 (internal sequence) (Cysteine residue).
Database link: O60481 -
Positive control
- Human Prostate tissue
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
---|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. -
Involvement in disease
Heterotaxy, visceral, 1, X-linked
VACTERL association X-linked with or without hydrocephalus
Congenital heart defects, multiple types, 1, X-linked -
Sequence similarities
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Domain
The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation. -
Cellular localization
Nucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression (By similarity). Translocation to the nucleus requires KPNA1 or KPNA6. - Information by UniProt
-
Database links
- Entrez Gene: 7547 Human
- Entrez Gene: 22773 Mouse
- Entrez Gene: 367944 Rat
- Omim: 300265 Human
- SwissProt: O60481 Human
- SwissProt: Q62521 Mouse
- Unigene: 111227 Human
- Unigene: 255890 Mouse
-
Alternative names
- Heterotaxy 1 antibody
- HTX antibody
- HTX1 antibody
see all
Images
Datasheets and documents
-
Datasheet download
References (0)
ab189969 has not yet been referenced specifically in any publications.