The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionActs as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Involvement in diseaseHeterotaxy, visceral, 1, X-linked VACTERL association X-linked with or without hydrocephalus Congenital heart defects, multiple types, 1, X-linked
Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers.
DomainThe C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.
Cellular localizationNucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression (By similarity). Translocation to the nucleus requires KPNA1 or KPNA6.