Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)
- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-Zinc Alpha 2 Glycoprotein antibody
See all Zinc Alpha 2 Glycoprotein primary antibodies -
DescriptionRabbit polyclonal to Zinc Alpha 2 Glycoprotein
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Zinc Alpha 2 Glycoprotein.
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Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Liver; Human Plasma Total Protein; Human Heart; Human Lung; Human Adipose. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: HeLa.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab117275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa). |
Target
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FunctionStimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.
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Tissue specificityBlood plasma, seminal plasma, urine, saliva, sweat, epithelial cells of various human glands, liver.
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Sequence similaritiesBelongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 563 Human
- Entrez Gene: 12007 Mouse
- Entrez Gene: 25294 Rat
- Omim: 194460 Human
- SwissProt: P25311 Human
- SwissProt: Q64726 Mouse
- SwissProt: Q63678 Rat
- Unigene: 546239 Human
see all -
Alternative names
- Alpha 2 glycoprotein 1 zinc antibody
- Alpha 2 glycoprotein 1 zinc binding antibody
- Alpha 2 glycoprotein zinc antibody
see all
Images
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Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human Plasma Total Protein Lysate
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lane 4 : Lung (Human) Tissue Lysate
Lane 5 : Human adipose normal tissue lysate - total protein (ab28980)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 44 kDa (why is the actual band size different from the predicted?)
Additional bands at: 100 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
Zinc Alpha 2 Glycoprotein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)ICC/IF image of ab117275 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab117275 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)IHC image of ab117275 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117275, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References
This product has been referenced in:
- Liu J et al. AZGP1 inhibits soft tissue sarcoma cells invasion and migration. BMC Cancer 18:89 (2018). WB, IHC-P ; Human . Read more (PubMed: 29357838) »
- Philley JV et al. Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease. Oncotarget 8:18070-18081 (2017). Read more (PubMed: 28160560) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"