ab38929 (4µg/ml) staining MMP1 - Hemopexin domain in human gallbladder using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of epithelial cells. Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Western blot - Zinc Alpha 2 Glycoprotein antibody (ab47116)
All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody (ab47116) at 1 µg/ml
Lane 1 : Human Plasma Total Protein Lysate Lane 2 : Human liver tissue lysate - total protein (ab29889) Lane 3 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution developed using the ECL technique