Recombinant Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14425(B)] to Zinc Alpha 2 Glycoprotein
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)]
See all Zinc Alpha 2 Glycoprotein primary antibodies -
Description
Rabbit monoclonal [EPR14425(B)] to Zinc Alpha 2 Glycoprotein -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human Plasma and HepG2 cell lines; Human liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14425(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 34 kDa).
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IP |
1/30.
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IHC-P |
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 34 kDa). |
IP
1/30. |
IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids. -
Tissue specificity
Blood plasma, seminal plasma, urine, saliva, sweat, epithelial cells of various human glands, liver. -
Sequence similarities
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 563 Human
- Omim: 194460 Human
- SwissProt: P25311 Human
- Unigene: 546239 Human
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Alternative names
- Alpha 2 glycoprotein 1 zinc antibody
- Alpha 2 glycoprotein 1 zinc binding antibody
- Alpha 2 glycoprotein zinc antibody
see all
Images
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Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/20000 dilution + Human plasma at 20 µg
Secondary
Anti-rabbit (HRP) specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 34 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/2000 dilution + HepG2 cell lysate at 10 µg
Secondary
Goat Anti-rabbit HRP at 1/1000 dilution
Predicted band size: 34 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Immunoprecipitation of Human plasma labeling Zinc Alpha 2 Glycoprotein using ab180574at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500.
Blocking/dilution buffer and concentration: 5% NFDM/TBST
All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/30 dilution
Lane 1 : Human plasma
Lane 2 : Negative control
Secondary
All lanes : Anti-Rabbit (HRP) specific to the non-reduced form of IgG at 1/500 dilution -
Immunohistochemical analysis of Human liver tissue labeling Zinc Alpha 2 Glycoprotein with ab180574 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab180574 has been referenced in 2 publications.
- Fu X et al. Molecular mechanism of albumin in suppressing invasion and metastasis of hepatocellular carcinoma. Liver Int 42:696-709 (2022). PubMed: 34854209
- Xue Y et al. Zinc-a-2-glycoprotein: a candidate biomarker for colon cancer diagnosis in Chinese population. Int J Mol Sci 16:691-703 (2015). WB ; Human . PubMed: 25561225