Recombinant
RabMAb

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)

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Overview

  • Product name
    Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)]
    See all Zinc Alpha 2 Glycoprotein primary antibodies
  • Description
    Rabbit monoclonal [EPR14425(B)] to Zinc Alpha 2 Glycoprotein
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Zinc Alpha 2 Glycoprotein aa 50-150. The exact sequence is proprietary.
    Database link: P25311

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human Plasma and HepG2 cell lines; Human liver tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR14425(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 34 kDa).
IP 1/30.
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.
  • Tissue specificity
    Blood plasma, seminal plasma, urine, saliva, sweat, epithelial cells of various human glands, liver.
  • Sequence similarities
    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • Alpha 2 glycoprotein 1 zinc antibody
      • Alpha 2 glycoprotein 1 zinc binding antibody
      • Alpha 2 glycoprotein zinc antibody
      • Azgp1 antibody
      • ZA2G antibody
      • ZA2G_HUMAN antibody
      • ZAG antibody
      • Zinc alpha 2 glycoprotein precursor antibody
      • Zinc-alpha-2-glycoprotein antibody
      • Zn alpha 2 glycoprotein antibody
      • Zn alpha 2 GP antibody
      • Zn alpha2 glycoprotein antibody
      • Zn-alpha-2-glycoprotein antibody
      • Zn-alpha-2-GP antibody
      • ZNGP1 antibody
      see all

    Images

    • Western blot - Anti-Zinc Alpha 2 Glycoprotein [EPR14425(B)] antibody (ab180574)
      Western blot - Anti-Zinc Alpha 2 Glycoprotein [EPR14425(B)] antibody (ab180574)
      Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/20000 dilution + Human plasma at 20 µg with NFDM/TBST at 5 %

      Secondary
      Anti-rabbit (HRP) specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 34 kDa

    • Western blot - Anti-Zinc Alpha 2 Glycoprotein [EPR14425(B)] antibody (ab180574)
      Western blot - Anti-Zinc Alpha 2 Glycoprotein [EPR14425(B)] antibody (ab180574)
      Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/2000 dilution + HepG2 at 10 µg with NFDM/TBST at 5 %

      Secondary
      Goat Anti-rabbit HRP at 1/1000 dilution

      Predicted band size: 34 kDa

    • Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein [EPR14425(B)] antibody (ab180574)
      Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein [EPR14425(B)] antibody (ab180574)

      Immunoprecipitation of Human plasma labeling Zinc Alpha 2 Glycoprotein using ab180574at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST



      All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/30 dilution

      Lane 1 : Human plasma with NFDM/TBST
      Lane 2 : Negative control with NFDM/TBST

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : Anti-Rabbit (HRP) specific to the non-reduced form of IgG at 1/500 dilution
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein [EPR14425(B)] antibody (ab180574)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein [EPR14425(B)] antibody (ab180574)

      Immunohistochemical analysis of Human liver tissue labeling Zinc Alpha 2 Glycoprotein with ab180574 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.

    References

    This product has been referenced in:
    • Xue Y  et al. Zinc-a-2-glycoprotein: a candidate biomarker for colon cancer diagnosis in Chinese population. Int J Mol Sci 16:691-703 (2015). WB ; Human . Read more (PubMed: 25561225) »

    See 1 Publication for this product

    Publishing research using ab180574? Please let us know so that we can cite the reference in this datasheet.

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