Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab65767)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab65767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation.
  • Tissue specificity
    Expressed most abundantly in ovary and, at lower levels, in prostate, spleen and testis. Weak expression, if any, in thymus, small intestine, colon and peripheral blood leukocytes.
  • Sequence similarities
    Contains 1 SP-RING-type zinc finger.
  • Domain
    The C-terminal proline-rich domain possesses a significant intrinsic transcriptional activity. This activity is inhibited by the N-terminus in the full-length protein.
  • Cellular localization
    Nucleus speckle. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • hZIMP10 antibody
    • KIAA1224 antibody
    • MIZ antibody
    • PIAS like protein hZimp10 antibody
    • PIAS like protein on chromosome 10 antibody
    • PIAS like protein Zimp10 antibody
    • PIAS-like protein Zimp10 antibody
    • RAI 17 antibody
    • Retinoic acid induced 17 antibody
    • Retinoic acid induced protein 17 antibody
    • Retinoic acid-induced protein 17 antibody
    • TRAFIP10 antibody
    • Zimp10 antibody
    • Zinc finger containing Miz1 PIAS like protein on chromosome 10 antibody
    • Zinc finger MIZ domain-containing protein 1 antibody
    • Zinc finger MIZ-type containing 1 antibody
    • Zmiz1 antibody
    • ZMIZ1_HUMAN antibody
    see all

Images

  • Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab65767) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 115 kDa
    Observed band size : 115 kDa
    Additional bands at : 107 kDa (possible isoform).

    Exposure time : 15 minutes
  • ICC/IF image of ab65767 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65767, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.

References

ab65767 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab65767.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up