Recombinant
RabMAb

Anti-Zinc finger protein 460 antibody [EPR13602] (ab182010)

Overview

  • Product name
    Anti-Zinc finger protein 460 antibody [EPR13602]
    See all Zinc finger protein 460 primary antibodies
  • Description
    Rabbit monoclonal [EPR13602] to Zinc finger protein 460
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human Zinc finger protein 460 aa 1-150. The exact sequence is proprietary.
    Database link: Q14592

  • Positive control
    • LnCap cells. HepG2, BxPC-3, LnCap and LoVo whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR13602
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab182010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 64 kDa.
ICC/IF 1/500.

Target

  • Function
    May be involved in transcriptional regulation.
  • Tissue specificity
    Ubiquitously expressed at low levels. Highest levels are found in pancreas and liver.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 11 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HZF 8 antibody
    • HZF8 antibody
    • Zinc finger protein 272 antibody
    • Zinc finger protein 460 antibody
    • Zinc finger protein HZF8 antibody
    • ZN460_HUMAN antibody
    • ZNF 272 antibody
    • ZNF 460 antibody
    • ZNF272 antibody
    • ZNF460 antibody
    see all

Images

  • All lanes : Anti-Zinc finger protein 460 antibody [EPR13602] (ab182010) at 1/10000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : BxPC-3 whole cell lysate
    Lane 3 : LnCaP whole cell lysate
    Lane 4 : LoVo whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 64 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunofluorescent analysis of formaldehyde fixed LnCap cells labeling Zinc finger protein 460 using ab182010 at a 1/500 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution.

References

ab182010 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab182010.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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